The mechanism of clot dissolution by plasmin.
نویسندگان
چکیده
منابع مشابه
Flow rate-modulated dissolution of fibrin with clot-embedded and circulating proteases.
The efficiency of plasmin, miniplasmin, and neutrophil leukocyte elastase in fibrin digestion is well characterized in static systems. Since in vivo the components of the fibrinolytic system are permanently exposed to flow, we have developed two in vitro models and studied the effect of shear forces on fibrin dissolution with these proteases. Cylindrical nonocclusive fibrin clots are perfused a...
متن کاملPlasmic Degradation of Crosslinked Fibrin. I. Structural Analysis of the Particulate Clot and Identification of New Macromolecular Solub1e Complexes
Plasmic degradation of crosslinked fibrin has been studied to identify the proteolytic cleavages that convert the clot into a soluble lysate and also to identify the derivatives that are likely to circulate during clot dissolution. Initial polypeptide chain cleavages do not disrupt the solid clot matrix. With continued exposure to plasmin, high molecular weight derivatives are produced that rem...
متن کاملPlasmic degradation of crosslinked fibrin. I. Structural analysis of the particulate clot and identification of new macromolecular-soluble complexes.
Plasmic degradation of crosslinked fibrin has been studied to identify the proteolytic cleavages that convert the clot into a soluble lysate and also to identify the derivatives that are likely to circulate during clot dissolution. Initial polypeptide chain cleavages do not disrupt the solid clot matrix. With continued exposure to plasmin, high molecular weight derivatives are produced that rem...
متن کاملThe mechanism of plasminogen activation and fibrin dissolution by single chain urokinase-type plasminogen activator in a plasma milieu in vitro.
The relative contribution of several mechanisms to plasminogen activation and fibrin dissolution by urokinase-type plasminogen activator (u-PA) in vitro was quantitated. The activation of plasminogen by recombinant single chain u-PA (rscu-PA), by its two chain derivative (rtcu-PA) and by a plasmin-resistant mutant, rscu-PA-Glu158, obeys Michaelis-Menten kinetics with catalytic efficiencies of 0...
متن کاملProteolytic activity determined with a substrate tagged with radioactive iodine.
The proteolytic enzyme, plasmin, is occasionally found in the plasma of patients with a variety of pathologic conditions. Its presence is detected by observing the complete dissolution of the clot of fibrin (fibrinolysis) formed by the coagulation of blood (1). Methods based on time of fibrinolysis are unsuitable for detecting quantities of plasmin that are insufficient to produce complete lysi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of clinical investigation
دوره 38 7 شماره
صفحات -
تاریخ انتشار 1959